anti hdac6 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti hdac6
Anti Hdac6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hdac6/HDAC6+Rabbit+mAb/10__1038_slash_s41467___026___77932___z-507-7-8
Average 95 stars, based on 129 article reviews

anti hdac6 - by Bioz Stars, 2026-11

95/100 stars

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Article Title: DCLK1 drives malignant progression and chemoresistance of bladder cancer by deubiquitinating HDAC6.
Article Snippet: cDNA amplification was performed using Platinum SYBR Green qPCR Super Mix-UDG (Invitrogen) on a CFX96 Touch system (Bio-Rad), with tubulin as the reference control.

Article Title: Functional landscape of ubiquitin linkages couples K29-linked ubiquitylation to epigenome integrity
Article Snippet: The following primary antibodies were used: Actin (MAB1501, Millipore (RRID:AB_2223041)); GAPDH (sc-20357, Santa Cruz (RRID:AB_641107)); GFP (11814460001, Merck (RRID:AB_390913)); HA (11867423001, Roche (RRID;AB_390918)), FLAG (F1804, Sigma (RRID:AB_262044)); RPS27A (ab111598, Abcam (RRID:AB_10863285)); Tubulin (T9026, Sigma (RRID:AB_2541185)); UBA52 (PA5-23685, Thermo (RRID:AB_2541185)); Ub (FK2) (BML-PW8810, Enzo (RRID:AB_2820835)); Ub (P4D1) (sc-8017, Santa Cruz (RRID:AB_2762364)); Ub K11-linked (SAB5701121, Sigma); Ub K27-linked (ab181537, Abcam (RRID:AB_2713902)); Ub K33-linked (PA5-120623, Thermo (RRID:AB_2914195)); Ub K48-linked (Apu2) (05-1307, Sigma (RRID:AB_11213655)); Ub K63-linked (BML-PW0600-0025, Enzo (RRID:AB_2052278)); SUV39H1 (8729 T, Cell Signaling (RRID:AB_10829612)); SUV39H2 (LS-C116360, Ls Bio (RRID:AB_10914491)); TRABID (ab262879, Abcam); TRIP12 (A301-814A, Bethyl Labs (RRID:AB_1264344)); JMJD1B (2621S, CST (RRID:AB_915946)); JMJD2A (NB110-40585, Novus (RRID:AB_669535)); RNF168 (kind gift from Daniel Durocher (Stewart et al, )); NDC80 (kind gift from Jakob Nilsson; raised against NDC80 aa1-230 by Moravian Biotechnology); H3K9me3 (ab176916, Abcam (RRID:AB_2797591) and 61013, Thermo (RRID:AB_2687870)); SETDB1 (ab107225, Abcam (RRID:AB_10861045)); BRD4 (13440, CST (RRID:AB_2687578)); Histone H3-pS10 (06-570, Merck (RRID:AB_310177)); 53BP1 (MAB3802, Millipore (RRID:AB_11212586)); γH2AX (05-636, Millipore (RRID:AB_309864)); CISD1 (NBP2-92696, Novus (RRID:AB_3462596)); CWC27 (NBP1-82508, Novus (RRID:AB_11030884)); NSFL1C (NBP2-13677, Novus (RRID:AB_3261294)); XPC (A301-122 A, Bethyl (RRID: AB_2288476)); HDAC6 (7558, CST (RRID:AB_10891804)); TOPORS (Liu et al, ); RGS17 (12549-1-AP, Proteintech (RRID:AB_2179954)).

Incubation:

Article Title: Artemisinin-derived hydrophobic tags: structural modification and structure-activity relationship exploration of HDAC6/1 targeting degraders with potent antileukemic efficacy.
Article Snippet: Hydrophobic tags (HyTs) in targeted protein degradation (TPD) offer a novel strategy to address HDAC off-target effects and drug resistance in hematological cancers.. Artemisinin derivatives,characterized by their unique and highly hydrophobic endoperoxide bridge, emerge as promising candidates for HyTs development.. However, the structure-activity relationship has not been systematically investigated.

Blocking Assay:

Article Title: Primary cilia promote resistance to EGFR tyrosine kinase inhibitor, osimertinib, in non–small cell lung cancer
Article Snippet: Proteins were separated via 4-20% gradient SDS-PAGE (Bio-Rad), transferred to membranes, and blocked with 5% bovine serum albumin (Sigma-Aldrich). .. After the blocking, the membranes were immunoblotted with one of these primary antibodies: HDAC6 (CST, Cat no. 7558, 1:1000), acetylated-α-Tubulin (Sigma, Cat no. T6793, 1:1000), GAPDH (CST, Cat no. 5174, 1:1000), or tubulin (Santa Cruz Biotechnology, sc-5286, 1:500). ..

Western Blot:

Article Title: PACS1 syndrome mutation disrupts dynein-mediated cargo transport via HDAC6 and BICD2
Article Snippet: .. Antibodies: Antibodies against actin (Sigma, MAB1501, 1:5000), AP1M1 (ABclonal, A10129, 1:1000), α/β-tubulin (Cell Signaling Technology (CST), 2148S 1:5000), V5 (Invitrogen, R960-25, 1:2000), FLAG (for WB: Sigma‒Aldrich, F7425, 1:5000 and for IP: Sigma‒Aldrich, A2220, 50% slurry), HA (for WB: CST 3724S, 1:4000; ABclonal AE026, 1:4000; Invitrogen MA1-12429, 1:4000; for IF: Santa Cruz sc-7392, 1:100, Biolegend #901513, 1:800), GFP (Invitrogen, MA5-15256, 1:2000), furin clone MON-152 (Enzo Life Sciences, ALX-803-017, 1:100), Giantin (kindly provided by Dr. A. Linstedt, CMU, 1:750), PACS1 (for IP: Ab 702, 1:100 and for WB: Ab703 , 1:1000), PACS2 (Ab 193 , 1:1000), cytoplasmic dynein heavy chain (Sigma ABT266, 1:1000), Dynein intermediate chain 74.1 (Invitrogen, MA1-070, 1:1000), p150 Glued (CST, 69399, 1:1000), HOOK1 (Proteintech, 10871-1-AP, 1:1000), MIRO1 (also known as RHOT1, ABclonal, A5838, 1:1000), KIF5B (CST, 18148S, 1:1000), BICD2 (Sigma, SAB2702337, 1:1000), LAMTOR1 (CST 8975 T, 1:100), Lis1 (Invitrogen, PA5-20419, 1:1000), DNA-PKcs (CST 4602S, 1:1000), Actinin (CST 3134S, 1:1000), α-tubulin (CST 3873S, 1:1000), Acetyl-α-tubulin (CST 5335, 1:1000), HDAC6 (CST 7558S, 1:1000), Rabbit IgG, whole molecule (Jackson ImmunoResearch Lab., 011-000-003, 1:100). .. Chemicals: DMSO (Sigma, 2650), ribociclib (Tocris, 7050), Ro-3306 (Tocris, 4181), rapalog (Takara Bio, 635057), and tubacin (Cayman Chemical, 13691-1).

Article Title: PACS1 syndrome mutation disrupts dynein-mediated cargo transport via HDAC6 and BICD2.
Article Snippet: Antibodies: Antibodies against actin (Sigma, MAB1501, 1:5000), AP1M1 (ABclonal, A10129, 1:1000), α/β-tubulin (Cell Signaling Technology (CST), 2148S 1:5000), V5 (Invitrogen, R960- Communications Biology | (2026) 9:450 11 25, 1:2000), FLAG (for WB: Sigma‒Aldrich, F7425, 1:5000 and for IP: Sigma‒Aldrich, A2220, 50% slurry), HA (for WB: CST 3724S, 1:4000; ABclonal AE026, 1:4000; Invitrogen MA1-12429, 1:4000; for IF: Santa Cruz sc-7392, 1:100, Biolegend #901513, 1:800), GFP (Invitrogen, MA515256, 1:2000), furin cloneMON-152 (Enzo Life Sciences, ALX-803-017, 1:100), Giantin (kindly provided byDr. .. A. Linstedt, CMU, 1:750), PACS1 (for IP: Ab 702, 1:100 and for WB: Ab70345, 1:1000), PACS2 (Ab 19316, 1:1000), cytoplasmic dynein heavy chain (Sigma ABT266, 1:1000), Dynein intermediate chain 74.1 (Invitrogen,MA1-070, 1:1000), p150Glued (CST, 69399, 1:1000), HOOK1 (Proteintech, 10871-1-AP, 1:1000), MIRO1 (also known as RHOT1, ABclonal, A5838, 1:1000), KIF5B (CST, 18148S, 1:1000), BICD2 (Sigma, SAB2702337, 1:1000), LAMTOR1 (CST 8975 T, 1:100), Lis1 (Invitrogen, PA5-20419, 1:1000), DNA-PKcs (CST 4602S, 1:1000), Actinin (CST 3134S, 1:1000), α-tubulin (CST 3873S, 1:1000), Acetyl-α-tubulin (CST 5335, 1:1000), HDAC6 (CST 7558S, 1:1000), Rabbit IgG, whole molecule (Jackson ImmunoResearch Lab., 011-000-003, 1:100). .. Chemicals: DMSO (Sigma, 2650), ribociclib (Tocris, 7050), Ro-3306 (Tocris, 4181), rapalog (Takara Bio, 635057), and tubacin (Cayman Chemical, 13691-1).

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